Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 9 de 9
Filter
1.
Bol. micol. (Valparaiso En linea) ; 35(2): 2-8, dic. 2020. tab, ilus
Article in Spanish | LILACS | ID: biblio-1437200

ABSTRACT

Determinamos los géneros de hongos anamorfos que contaminan los libros del área de cuarentena y limpieza, dentro del Área Histórica de la Universidad Central del Ecuador (UCE). Realizamos un hisopado aleatorio a una muestra representativa de 50 de estos libros de acuerdo a una Tabla militarizada estándar. También hisopamos como muestra preferencial a 21 libros gravemente contaminados con hongos. Los hisopados tuvieron una superficie de 5x5 cm, friccionando en la pasta, el borde y el interior de estos libros. Las 213 muestras tomadas fueron inoculadas en medio de cultivo Agar Malta. Los medios fueron incubados a una temperatura de 28°C durante 7 días. Realizamos observaciones por microscopía a 40 y 100x además de usar literatura especializada para la identificación hasta el nivel de género de hongos anamorfos. Los géneros más abundantes en este estudio fueron Penicillium (80,2%) y Mucor (8,1%). (AU)


We determined the genera of anamorphic fungi that contaminate the books in the quarantine and cleaning area, within the Historical Area of the Central University of Ecuador (CUE). We performed a random swab on a representative sample of 50 of these books according to a standard militarized Table. We also swabbed as a preferential sample 21 books seriously contaminated with fungi. The swabs had a surface area of 5x5 cm, rubbing on the paste, the edge and the interior of these books. The 213 samples taken were inoculated in Agar Malta culture medium. The media were incubated at a temperature of 28° C for 7 days. We made observations by microscopy at 40 and 100x in addition to using specialized literature for the identification down to the genus level of anamorphic fungi. The most abundant genus in this study were Penicillium(80,2%) and Mucor(8,1%). (AU)


Subject(s)
Penicillium/isolation & purification , Mucor/isolation & purification , Penicillium/pathogenicity , Colony Count, Microbial/methods , Mitosporic Fungi/pathogenicity , Ecuador , Libraries, Special
2.
Hig. aliment ; 33(288/289): 2793-2797, abr.-maio 2019. graf, tab
Article in Portuguese | LILACS, VETINDEX | ID: biblio-1482339

ABSTRACT

Este trabalho avaliou o efeito antifúngico in vitro de toxina killer de Hansenula wingei contra Aspergillus ochraceus e Penicillium expansum deteriorantes de alimentos. O extrato livre de células-ELC contendo a toxina killer (obtido a partir do cultivo da levedura em Caldo MPL a 25ºC/96 horas) foi submetido ao ensaio antifúngico por meio de análise microscópica, determinando-se a porcentagem de germinação conidial e o desenvolvimento de hifas dos fungos testados. H. wingei inibiu a germinação conidial de A. ochraceus e P. expansum em 98,91% e 96,49%, respectivamente, bem como a inibição do desenvolvimento micelial de ambos os fungos foi maior que 78%. O composto antifúngico mostrou-se estável ao tratamento térmico de 90ºC/30 min., indicando a possibilidade de aplicação no biocontrole in situ de frutos pós-colheita.


Subject(s)
Antifungal Agents/administration & dosage , Aspergillus ochraceus/pathogenicity , Fruit , Fungi , Yeasts , Penicillium/pathogenicity , Pichia
3.
Rev. cuba. med. gen. integr ; 26(4): 647-655, oct.-dic. 2010.
Article in Spanish | LILACS | ID: lil-584863

ABSTRACT

Las enfermedades alérgicas y el asma, sobre todo en la población infantil, son afecciones muy frecuentes en todo el mundo y en Cuba. Con el objetivo de determinar la frecuencia de sensibilización a hongos ambientales y su relación con enfermedades atópicas, se realizó un estudio analítico transversal en 100 escolares de 6 a 7 años de una escuela primaria de San Antonio de los Baños, provincia La Habana, en el período comprendido entre septiembre de 2006 y marzo de 2007. El 27 por ciento de la muestra seleccionada padecía de asma, el 40 por ciento de rinitis alérgica y el 26 por ciento de dermatitis atópica. La sensibilización micótica más frecuente resultó Penicillium, para un 50 por ciento de la muestra. No hubo una asociación estadísticamente significativa entre reactividad cutánea a hongos ambientales y la presencia de enfermedades atópicas; tampoco fue significativa su asociación con la enfermedad alérgica respiratoria ni dermatitis atópica. Se concluye que la mayor sensibilización a hongos anemófilos se apreció al Penicillium, aunque sin asociación con la presencia de enfermedades atópicas


The allergic diseases and the asthma, mainly in children, are very frequent affections at world level and in Cuba. The objective of present paper was to determine the sensitization frequency to environmental fungi and its relation to atopic diseases, thus, a cross-sectional and analytical study was conducted in 100 school children aged 6 to 7 from a primary school of San Antonio de los Ba±os, La Habana province, from September 2006 to March, 2007. The 27 percent of the selected sample suffered from asthma, the 40 percent of allergic rhinitis, and the 26 percent had Penicillium for the 50 percent of sample. There was not a significant statistic association between the cutaneous reactivity to environmental fungi and the presence of atopic diseases and its association with the respiratory allergic disease as well as the atopic dermatitis was not significant. We conclude that the greater sensitization to anemophilic fungi was to Penicillium, although without an association with the presence of atopic diseases


Subject(s)
Humans , Male , Female , Child , Anti-Allergic Agents , Dermatitis, Atopic/epidemiology , Penicillium/pathogenicity , Cross-Sectional Studies , Skin Tests/methods
4.
Braz. j. microbiol ; 41(3): 700-706, Oct. 2010. ilus, graf, tab
Article in English | LILACS | ID: lil-549426

ABSTRACT

The aim of this research was the analysis of the possible antagonistic effect of Penicillium oxalicum over the pathogen rice fungus A. alternata under different conditions of temperature, water activity and culture media. The macroscopic study of the dual growth revealed that according to the Index of Dominance P. oxalicum was more competitive that A. alternata at 25ºC whereas at 15ºC was this species. Microscopic analysis showed that P. oxalicum was a mycoparasite of A. alternata at all conditions tested. The antagonist penetrated into A. alternata and disintegrated its conidiophores and conidia. The results suggests that P. oxalicum may be a possible biological control agent of the rice pathogens in a future.


Subject(s)
Alternaria/growth & development , Alternaria/isolation & purification , Oryza , Penicillium/pathogenicity , Food Samples , Methods , Methods , Virulence
5.
Bol. micol ; 14(1/2): 67-71, 1999. tab
Article in Spanish | LILACS | ID: lil-255769

ABSTRACT

Se cuantificó e identificó la población de hongos celulolíticos, asociados al rastrojo de trigo, relacionándolos con algunas variables ambientales. En parcelas bajo siembra directa del grano, incluidas en un diseño en bloques aleatorizados con tres repeticiones, los restos de cosecha de trigo obtenidos de 1 m(2) se cortaron y distribuyeron en bolsas de malla. Mensualmente se procedió a determinar la pérdida de peso del rastrojo y a registrar las variables agroclimáticas. Se cuantificó e identificó la población fúngica celulolítica por el método de dilución en placas en medio selectivo. Por análisis de regresión múltiple, se examinó la relación entre las variables ambientales y el peso del rastrojo con la micota celulolítica (r(2)= 0,95). Por análisis stepwise, la temperatura edáfica, la variación de la humedad relativa y las precipitaciones fueron las variables más explicativas. Los hongos celulolíticos más aislados fueron especies de penicillium y fusarium. En este último género se destaca la presencia de f. solani y f. graminearum, potenciales patógenos de cultivos vegetales


Subject(s)
Biodegradation, Environmental , Environment , Fungi/isolation & purification , Fusarium/pathogenicity , Penicillium/isolation & purification , Triticum/microbiology , Fungi/pathogenicity , Penicillium/pathogenicity , Soil Microbiology
6.
EJMM-Egyptian Journal of Medical Microbiology [The]. 1996; 6 (1): 55-72
in English | IMEMR | ID: emr-40943

ABSTRACT

The importance of opportunistic fungal pathogens in causing ocular infections is emphasized. A study was conducted over a period of two years [1993-1994] to survey the ophthalmic as well as atmospheric fungi in Dakhlia and Qalubia Governorates at lower Egypt. From 500 patients suffering from keratomycosis, 14 species belonging to 12 fungal genera other than Aspergillus and Penicillium were identified. 10 genera comprising 13 fungal species other than Aspergilli and Penicillia were isolated from 250 farm animals showing clinical eye affections. 23 fungal genera including 60 fungal species were recorded as air-spora at the site of sampling. Of these atmospheric fungi, Aspergillus and Penicillium comprises 21 and 13 species respectively. A total of 47 characters and character states for Penicillium and 54 for Aspergillus are selected from morphological and physiological data on 13 and 38 ophthalmic collections described as Penicillium and Aspergillus respectively in this study. The most diverse isolates are become a subject for further taxonomic analysis for grouping the closely related isolates. The identification is judged by using known reference strains isolated from the surrounding atmosphere. Results lead to the suggestion a synoptic key for the etiological agents of corneal ulcer disease belonging to the two genera. The highest Q.S. values in this study indicated on the high affinities between ophthalmic and atmospheric fungi. Exponent of rabbit infection revealed that 55. 2% and 25% of infected rabbits showed positive pathogenicity and precipitin tests respectively. Positive correlation between proteolytic activities of these fungi and their precipitin test as well as their clinical findings were proved


Subject(s)
Humans , Animals , Fungi/pathogenicity , Aspergillus/pathogenicity , Penicillium/pathogenicity , Corneal Ulcer/etiology
8.
Bol. micol ; 9(1/2): 39-47, jul.-dic. 1994. tab
Article in Spanish | LILACS | ID: lil-153183

ABSTRACT

Mediante la selección de 8 compuestos plásticos utilizados en el mercado, adquiridos a la firma Aldrich Química SRL (Alemania), se valoró su capacidad de ser asimiladas como única fuente de carbono in vitro, por 2 grupos de microhongos pertenecientes a los asco-deuteromycetes. Estos compuestos fueron: poliestireno, polivinilacetato, poliacrilonitrito, policaprolactona, polimetacrilato de metilo, polivinilcloruro, acetato de celulosa y polietileno. Solo la policaprolactona y el polivinilacetato fueron utilizados por los 2 grupos de hongos en estudio, en especial por cepas de aspergillus, chaetomiun, cladosporium, fusarium, penicillium y scopulariopsis. La policaprolactona fue el polímero más utilizado entre ph 6.5 a 8. Las diferentes especies pertenecientes a los géneros aspergillus y fusarium, presentaron la mayor actividad en la utilización de estos compusetos


Subject(s)
Aspergillus/pathogenicity , Biodegradation, Environmental , Chaetomium/pathogenicity , Cladosporium/pathogenicity , Fungi/pathogenicity , Fusarium/pathogenicity , In Vitro Techniques , Mitosporic Fungi/pathogenicity , Penicillium/pathogenicity , Plastics/chemical synthesis
9.
An. bras. dermatol ; 66(4): 185-9, jul.-ago. 1991. ilus
Article in Portuguese | LILACS | ID: lil-99939

ABSTRACT

Examinando, ao microscópico óptico comum, Penicillium marneffei Segretain, 1959, amostra isolada de um paciente francês com AIDS, apó seu retorno da Indonésia (Ancelle e dols), cultivada em ágar-Sabouraud a 37-C, após 14 dias de incubaçäo, verificamos ao lado de formas de frutificaçäo caracteristicas do gênero Penicillium, clamidoconídios de parede espessa, com septaçöes transversais formando dois a três elementos fúngicos. Estes foram semelhantes aos observados por Drouhet e cols. quando estudaram à microscopia eletrônica as formas parasitárias desse microrganismo no hamster dourado inoculado com a referida amostra. Justifica-se este trabalho para mostrar que, tanto em vida parasitária como em vida saprofítica os clamidoconídios com parede espessa e septos transversais ocorrem com freqüência, näo havendo brotamento como em casos de histoplasmose clássica, em sua forma parasitária. Graças a este tipo de reproduçäo, podemos separar a histoplasmose da peniciliose pelo Penicillium marneffei com a qual as duas infecçöes podem se confundir ao exame histopatológico. Na histoplasmose, os fungos näo se dividem por fissäo binária


Subject(s)
Humans , Mycoses/pathology , Penicillium/ultrastructure , Microscopy, Electron/methods , Penicillium/analysis , Penicillium/pathogenicity
SELECTION OF CITATIONS
SEARCH DETAIL